Fig. 5

The Hsp90ab1 overexpression promoted the growth and metastasis of GC in vivo. a–c Images of subcutaneous tumors of mice injected with Hsp90ab1-overexpression and control stable transfected cells. a MKN45 cells with upregulated Hsp90ab1 expression exhibited enhanced tumor growth in nude mice. b The effect of Hsp90ab1 on GC tumor growth was evaluated based on tumor volume injected with indicated cells. The data of subcutaneous tumors were expressed as mean ± SD. c Scatter plots of tumor weight derived from indicated cells after subcutaneous implantation. d, e The xenograft tumors was stained with H&E and the expression of Hsp90ab1 and Ki-67 in the tumors was measured by IHC. Proliferative ability was measured by the Ki-67 index (%). f–h Hsp90ab1 upregulated cells or control cells were injected into nude mice through the tail vein to evaluate the metastatic potential of cells. The infection efficacy of lentivirus with GFP in vivo (f) and number of metastatic peritoneal nodules (g) in individual mice was analyzed. h The lung metastasis and metastatic peritoneal nodules sections were stained with (H&E). Bottom pictures were local magnification of top pictures. *P < 0.05; **P < 0.01; ***P < 0.001