Fig. 6 | Oncogene

Fig. 6

From: Hsp90ab1 stabilizes LRP5 to promote epithelial–mesenchymal transition via activating of AKT and Wnt/β-catenin signaling pathways in gastric cancer progression

Fig. 6

Hsp90ab1 interacted with LRP5 protein in GC. a Immunoprecipitation assays were used to identify proteins associated with Hsp90ab1. The anti-Hsp90ab1 and IgG antibody were incubated with cell extracts, the bands specific were excised and submitted for mass spectrometry, and LRP5 was identified to be a Hsp90ab1-binding protein. b Co-IP of Hsp90ab1 and LRP5 with each other from proteins of BGC823 and MKN45 cells in GC cells. c Confocal immunofluorescence analysis showed the presence and localization of Hsp90ab1 and LRP5 in the cytoplasm of MKN45 cells. d, e Schematics outlines of both Hsp90ab1 and LRP5 structure features. f–h GST pull-down assay results indicated the direct interaction between Hsp90ab1 and LRP5. i Pull-down of LRP5 by GST-tagged Hsp90ab1 fragment (264–609a) and Hsp90ab1 fragment (1–263/610–724a)

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