Fig. 5 | Oncogene

Fig. 5

From: Cancer stem-like properties and gefitinib resistance are dependent on purine synthetic metabolism mediated by the mitochondrial enzyme MTHFD2

Fig. 5

MTHFD2-dependent growth of drug-resistant PC9M2 cells. a Western blot for MTHFD2 in gefitinib-sensitive PC9 cells compared with A549 cells and SAECs. b Western blot for MTHFD2 in MTHFD2 knockdown PC9 cells (upper panel). β-Actin was used as loading control. Cell proliferation assay with MTHFD2 knockdown PC9 cells (lower panel). NCT negative control; shMTHFD2#53, MTHFD2 knockdown (lower panel). Experiments were performed three times and the representative results were presented. The data are represented as mean ± SD, N = 3. c MTHFD2 protein (upper panel) and MTHFD2 mRNA (lower panel) expression levels in PC9 and gefitinib-resistant PC9M2 cells. The data are represented as mean ± SD, N = 3. d Western blot for MTHFD2 in MTHFD2 knockdown PC9M2 cells (upper panel). β-Actin was used as loading control. Cell proliferation assay with MTHFD2 knockdown PC9M2 cells (lower panel). Experiments were performed three times and the representative results were presented. The data are represented as mean ± SD, N = 3. Statistical significance is calculated with a two-tailed unpaired t-test; N.S. not significant; **p < 0.01

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