Fig. 2
From: Inhibitors of ribosome biogenesis repress the growth of MYCN-amplified neuroblastoma
Cell viability of neuroblastoma cell lines treated with quarfloxin or CX-5461. a Cell viability of neuroblastoma cell lines treated with an 8-log fold dose range of quarfloxin (left panel) or CX-5461 (right panel). Absolute half-maximal inhibitory concentrations (IC50 values) are shown in Table 1. b SHEP-TET21N cells were seeded in the presence (low MycN) or absence (high MycN) of 1 ug/mL doxycycline (dox). On the following day, cells were treated for 48 h with an 8-log fold change dose range of quarfloxin (left panel) or CX-5461 (right panel). IC50 values are shown in Table 1. Insert: WB showing MycN expression in absence (-dox) and in presence of dox ( + dox). M = marker. Numbers to left indicate MW in kDa. c Cell viability of IMR-32 cells transfected with siRNAs (siMYCN-1 and siMYCN-2) targeting MYCN or a negative control siRNA (siNC), and treated with 50 nM quarfloxin (left panel) or 75 nM CX-5461 (right panel) for 48 h. The viability of vehicle + respective siRNA was set to 100%, and quarfloxin and CX-5461 treated cells were normalized to their respective controls. DMSO and DMF are vehicle controls to quarfloxin and CX-5461, respectively. For a, b, c; cell viability was measured with the Alamar blue assay. The data represents the mean cell viability and SD of two individual experiments performed in duplicate. (***p ≤ 0.001; ****p ≤ 0.0001)
