Fig. 5
From: Inhibitors of ribosome biogenesis repress the growth of MYCN-amplified neuroblastoma
Analysis of MycN and pre-rRNA expression in neuroblastoma cells treated with quarfloxin and CX-5461. a The protein levels of MycN are depleted in neuroblastoma cells with functional p53 upon treatment with quarfloxin or CX-5461. Representative western blot showing MycN protein levels in wt-TP53 IMR-32 and CHP-134 (upper panel) and TP53-mutated Kelly and BE(2)-C (lower panel) cells after 48 h treatment with 150 nM quarfloxin or 230 nM CX-5461. b Expression of 47S-rRNA (pre-rRNA) in IMR-32 cells treated for 24 h with low doses of quarfloxin (150 nM) and CX-5461(230 nM). c Expression of 47S-rRNA (pre-rRNA) in IMR-32 cells treated for 24 h with high doses of quarfloxin (1500 nM) and CX-5461(2300 nM). ns, not significant; ****p ≤ 0.0001
