Fig. 3
Tamoxifen treatment suppresses mechanosensing and YAP activation in HSCs. a Representative trace that shows the decrease in the amplitude of oscillation of a bead attached to a cell that can sense external mechanical stimuli (mechanosensing). b Histogram shows relative bead displacement for the first and last pulse, n = 25 cells (control), 20 cells (tam) and 21 cells (tam and GPER antagonist). c Representative images for immunofluorescence staining of HSCs, scale bar 20 µm. The white arrow indicates YAP nuclear localization, whereas the yellow arrow shows reduced nuclear YAP localization. d Quantification of YAP nuclear/cytoplasmic ratio, 16 control cells and 16 tamoxifen cells. e qPCR levels of YAP downstream genes CTGF and ANKRD1, normalized to RPLP0 and relative to control, three biological samples analysed in three different experiments. f Correlation GPER/YAP/CTGF expressions from TCGA database. Data from 492 patients. t-test, *P < 0.05, **P < 0.01,***P < 0.001. Three experimental replicates in all cases
