Fig. 3

Transient siRNA and stable shRNA knockdown of PTBP1 in non-small cell lung cancer cells, NSCLC. a ddTRAP assay detecting telomerase activity post siRNA knockdown of PTBP1 in H1299, H2882, H920, and HCC1359 (50 cell equivalents, n = 3, Student’s t-test set at *p < 0.05 for significance, **p < 0.005). b, c shRNA’s targeting PTBP1 were used to generate stable knockdowns in H1299 and H920. Telomerase activity was detected using ddTRAP assay and plotted for H1299 in panel b and H920 in c (50 cell equivalents, n = 3, Student’s t-test, *p < 0.05 for significance). d Splicing of hTERT mRNA was detected and quantified using ddPCR. Spliced TERT, indicated in blue, represents all spliced variants for hTERT. Potential FL TERT represents the total steady state hTERT mRNAs minus the spliced variants. NS represents non-silencing control shRNAs (n = 3). Data are expressed as means and standard error of the mean where applicable. ddTRAP = Droplet digital TRAP