Fig. 2
From: Lineage-specific control of TFIIH by MITF determines transcriptional homeostasis and DNA repair
GTF2H1 is a direct transcriptional target of MITF. a Human GTF2H1 locus with MITF-binding consensus sequences (E1–3) and ChIP primer annealing sites (colored arrows). TSS transcriptional start site, ATG, start codon. b ChIP, in vivo occupancy of MITF at GTF2H1 promoter in 501 mel cells. E box enrichment compared to intron 1. Mean ± SD from technical triplicates is presented. IgG (AB) was used as control antibody. c Normalized luciferase activity of GTF2H1 promoter after transfection with wild-type MITF vs. empty vector (EV) ± forskolin in A375 melanoma cells. Graphs represent mean ± SD from technical triplicates. d, e Normalized luciferase activity of GTF2H1 promoter containing E box site mutations in B16V or 501 mel cells ± forskolin. Mean ± SD from technical triplicates. b–e Experiments were performed twice with comparable results. f Transactivation of GTF2H1 promoter after wild-type MITF vs. SUMOylation-defective MITF-E318K transfection of HEK 293T cells as mean of fold-induction ± SD from 10 independent experiments (biological replicates). Right panel: immunoblot showing MITF protein levels in transfected HEK 293T cells. b–f *, p < 0.05; **, p < 0.01; ***, p < 0.001; two-tailed unpaired t-test)
