Fig. 1
Dynamic m6A modification patterns during chemical-induced cells transformation. a Metagene profiles of m6A distribution across the transcriptome in control and carcinogen induced cells. b Distribution of m6A peaks in the 5′-UTR (pink), coding sequence (CDS, blue), stop code(green), and 3′-UTR (red) of RNA transcripts. Representative charts show the proportion of m6A peaks in the indicated regions in SV-HUC-1 cells. c Consensus sequence motif for m6A methylation identified in SV-HUC-1 cells. d Overlap of genes with upregulated m6A modifications in the chemical-transformed cells. e Overlap of genes with downregulated m6A modifications in the transformed cells. f Gene Ontology Biological Process (GOBP) and Enrichment Analysis of genes with differential m6A modifications in control SV-HUC-1 vs. Cd, MCA-induced SV-HUC-1 transformed cells. (I) Analysis using 190 genes with upregulated m6A peaks in both Cd and MCA-induced transformed cells; (II) analysis using 90 genes with downregulated m6A peaks in both Cd and MCA-induced transformed cells. Each corresponding set of targets was subjected to DAVID GOBP analysis and an enrichment map was built by Cytoscape with Enrichment Map Apps. Each node denotes one enriched GOBP pathway (p < 0.005, FDR q < 0.1, overlap cutoff > 0.5), with its color reflecting the p-value. Node size is proportional to the total number of genes in each pathway. GOBP pathways with similar function were sorted into one cluster, marked with circles and labels. G representative differentially regulated m6A of CDCP1 mRNA in chemical transformation; IP immunoprecipitation
