Fig. 5

The role of the CCL5 pathway in macrophage migration. a Migration assays showing that macrophage migration was significantly reduced towards GH3-Aip-KD cell-derived conditioned media compared to GH3-NT control cell-derived conditioned media. Macrophages were in vitro treated with maraviroc (200 nM) for 24 h and the migration in response to GH3-Aip-KD-conditioned media and GH3-NT conditioned media was evaluated. Cells were counted in nine random fields and data are presented as mean ± SEM, n = 3. Graph showing the percentage of the MVC-treated migrated macrophages towards the GH3-NT and GH3-Aip-KD-conditioned medium relative to the vehicle. V vehicle, MVC maraviroc. P values indicated ***, <0.001; t-test. b Immunohistochemical analysis of CCL5 in normal pituitary (NP, n = 11), AIP-mutation-positive somatotroph adenomas (AIPpos GH, n = 12) and sporadic somatotroph adenomas (Sp GH, n = 17). Graph showing that CCL5 is highly upregulated in AIPpos tumors compared to the normal pituitary (left panel). Right panels show the representative images of CCL5 staining. P values indicated **, <0.01; one-way ANOVA with Bonferroni multiple comparison test. All images are ×200 magnification and scale bar = 100 µm. c Immunohistochemical analysis of FLI1 in normal pituitary (NP, n = 11), AIP-mutation-positive somatotroph adenomas (AIPpos GH, n = 12) and sporadic somatotroph adenomas (Sp GH, n = 17). Graph showing that FLI1 is highly upregulated in AIPpos tumors compared to the normal pituitary (left panel) and Sp GH tumors (left panel). Right panels show the representative images of FLI1 staining. P values indicated ***, <0.001; one-way ANOVA with Bonferroni multiple comparison test. All images are ×200 magnification and scale bar = 100 µm