Fig. 3 | Oncogene

Fig. 3

From: The role of microRNA-148a and downstream DLGAP1 on the molecular regulation and tumor progression on human glioblastoma

Fig. 3The alternative text for this image may have been generated using AI.

DLGAP1 was a direct target gene of miR-148a. a qRT-PCR analysis of DLGAP1 mRNA expression in normal astrocytes (HA1800) and GBM cells (U251, U87, T98G, and BT325) (*P < 0.05). b Western blotting analysis of DLGAP1 protein expression in normal astrocytes (HA1800) and GBM cells (U251, U87, BT325, and T98G) (*P < 0.05). c Representative images of DLGAP1 immunohistochemistry in normal brain and glioma tissues. Statistical analysis indicated that DLAGP1 was downregulated in glioma tissues compared with that in human normal brain tissues (*P < 0.05). d Schematics of predicted binding sites of miR-148a in the wild-type DLGAP1-3′-UTR and mutated sequence of DLGAP1-3′-UTR. Dual luciferase reporter assay showed that overexpression of miR-148a could significantly inhibit the luciferase activity of wild-type DLGAP1 3′-UTR, but unaffected the luciferase activity of mutant DLGAP1 3′-UTR (*P < 0.05). e, f Overexpression of miR-148a inhibited the expression of endogenous DLGAP1 and the inhibition of miR-148a promoted the expression of endogenous DLGAP1 mRNA and protein, as determined by qRT-PCR and western blotting (*P < 0.05). Error bars represent the mean ± SD of three independent experiments

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