Fig. 4 | Oncogene

Fig. 4

From: The role of microRNA-148a and downstream DLGAP1 on the molecular regulation and tumor progression on human glioblastoma

Fig. 4The alternative text for this image may have been generated using AI.

DLGAP1 mediated the effect of miR-148a on EMT in GBM. a EMT-associated proteins were regulated by silencing DLGAP1: downregulation of E-cad and upregulation of N-cad, vim, MMP2, and MMP9 at the protein level (*P < 0.05). b Wound-healing assays showed the downregulation of DLGAP1 increased GBM cell migration. c Tranwell assay showed that silencing DLGAP1 induced the U87 and U251 cells invasion (*P < 0.05). d Co-transfection of anti-miR-148a and shRNA-DLGAP1 partly restored the decrease in N-cad, vim, MMP2, and MMP9 mRNA caused by anti-miR-148a in both U87 and U251 cells, and the increase of E-cad caused by anti-miR-148a in U251 cells (*P < 0.05). e, f Wound-healing assays and Transwell assay. The inhibition of DLGAP1 partially reversed the effect of miR-148a inhibition on the migration and invasion of GBM cells. (*P < 0.05). Error bars represent the mean ± SD of three independent experiments

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