Fig. 4
The repression of CDKN1C mediated by mutant IDH1 is important for tumor progression. a Cell proliferation of CDKN1C-overexpressing JJ012 cells at the indicated times. The Y-axis shows the relative cumulative cell numbers. b (Left) Western blot analysis of CDKN1C in Cas9-expressing JJ012 cells electroporated with nontargeting crRNA or crRNA targeting CDKN1C and tracrRNA (#1 and #2). α-tubulin was used as a loading control. (Right) Bar graphs show the results of western blot quantification (n = 3). c Cell proliferation of CDKN1C-knockout JJ012 cells treated with 1 μM DS-1001b for 7 days. Approximately 104 cells were plated on a 6 cm dish and the cell number was set as day 0. The Y-axis shows the cumulative cell numbers. Data are expressed as the mean of triplicates (±s.d.). *P < 0.005, **P < 0.05 (Student’s t-test)
