Fig. 5
Pevonedistat treatment results in stabilization of p27 and induction of apoptosis in SI-NET cells. a Western blotting analysis of p27, cleaved PARP (cPARP), Cullin-1, and NEDD8 in NET cell lines after pevonedistat treatment at different concentrations for 72 h. Indicated by arrows in the NEDD8 blot are bands corresponding to neddylated cullin (~90 kDa) as well as unconjugated NEDD8 (~9 kDa). For all blots, GAPDH was used as loading control. b Apoptosis assay 72 h after pevonedistat treatment in NET cell lines. Representative propidium iodide (PI) and Annexin V-FITC flow cytometry plots are shown for DMSO-treated control cells and cells treated with 500 and 1000 nM pevonedistat. At the right, the proportion of cells positive for propidium iodide (PI) and Annexin V-FITC (AnnV) is shown (n:3)
