Fig. 6

Inhibition of MTH1 is associated with increased levels of oxidative DNA damage in EBV positive cells. Pairs of EBV-negative and -positive cells were treated with TH588 (10 µM) or S-Crizotinib (5 µM) for 24 h followed by immunofluorescence staining with antibodies against 8-oxodG (green) or γH2AX (red) and the nuclei were stained with DAPI (blue). a Representative microphotographs illustrating the expression of 8-oxodG and γH2AX in the untreated and treated BJAB/BJAB-B958 cell pair. b Representative microphotographs illustrating the expression of 8-oxodG and γH2AX in the untreated and treated BL41/BL41-E95B cell pair. c Quantification of the 8-oxoG specific fluorescence. Data are shown as the mean the ± SE of three independent experiments. **P < 0.01. d Percentage of γH2AX positive cells. Data are shown as the mean ± SE from three independent experiments. **P < 0.01