Fig. 1 | Oncogene

Fig. 1

From: Specific NOTCH1 antibody targets DLL4-induced proliferation, migration, and angiogenesis in NOTCH1-mutated CLL cells

Fig. 1

DLL4 is a potent stimulator of Notch pathway and proliferation in NOTCH1-mutated CLL. Cells from NOTCH1-mutated and -unmutated CLL cases were stimulated with Jagged1 (JAG1), Jagged2 (JAG2), DLL1, and DLL4 (10 μg/ml) ligands (a) or stromal cells OP9-JAG1, OP9-DLL1, and OP9-DLL4 (b). Cleaved Notch1 was analyzed by Western blot at 24 h and densitometrically quantified. β-Actin was probed as a loading control. Representative cases are shown (CLL 6, 9, and 18). c CFSE-labeled CLL cells were incubated with ODN-2006 and IL-15 together with JAG1, JAG2, DLL1, or DLL4. Reduction of CFSE fluorescence in viable CLL cells was quantified after 6 days by flow cytometry. Graph shows the percentage of cell proliferation induction of each ligand with respect to the unstimulated control. Mean ± SEM of all the samples analyzed (n = 6 NOTCH1-mutated and n = 6 NOTCH1-unmutated). **p < 0.01; *p < 0.05, ns not significant

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