Fig. 3: RNF181 correlates with ERα protein level in human samples and controls ERα target gene expression in whole genomic scale.
From: The ubiquitin ligase RNF181 stabilizes ERα and modulates breast cancer progression
a Examples of positive/negative RNF181, ERα, PR, and HER2 staining in breast tumor samples were shown by ×100 magnification. b RNF181 expression correlates with ERα level in human breast cancer samples. The correlation analysis between RNF181 expression and molecular/clinical characteristics in breast cancer samples was shown. P value < 0.05 was considered as statistical significance. c Top 10 signaling pathways significantly decreased/increased by RNF181 depletion in MCF7 cells. The pathway-enrichment analysis was used by the threshold P < 0.001 and fold change > 2 to derive regulated genes. RNF181 was depleted by siRNA (mix of siRNF181 #1 and siRNF181 #2) or treated with siControl. After 48 h, the whole mRNA was extracted for RNA sequence analysis. The siControl and siRNF181 were done in triplicates. d The heat-map graph shows the ERα regulating genes, which is significantly inhibited by RNF181 depletion in MCF-7 cells.
