Fig. 4: CDK phosphomotifs are unstable in the absence of Mastl.
From: The Greatwall kinase safeguards the genome integrity by affecting the kinome activity in mitosis
Motif enrichment (pLogo, [103]) for a all, b downregulated, and c upregulated phosphosites. d Gel separation of whole cell lysates from MastlFLOX or MastlNULL iMEFs collected at different cell-cycle phases (S, G2, and mitosis [M]) and immunoblotted with antibodies against the indicated proteins. MW Molecular weight ladder. Colored numbers: ph-CDK substrates pTPXK/R. Signal quantification of e the global signal or of f, g the indicated bands revealed by antibodies against phospho-CDK substrates depicted in d. A.U. Arbitrary unit. Error bars depict SD. pValue obtained from paired t-test on log2-transformed values from separate blots.
