Fig. 6: CDK1 and NEK2 target a large panel of phosphorylation sites affected by Mastl loss.
From: The Greatwall kinase safeguards the genome integrity by affecting the kinome activity in mitosis
a Cartoon depicting the process in order to test 101 downregulated phosphosites by radioative kinase assays using eight different kinases (see Figs. S7–S9). b Polar bar plot of the number of positive substrates for each kinase with their corresponding jitter plot of the mean of the ratio of the intensity for each susbstrate over a violin and box plot depicting their distribution. Red and orange dots indicate positively phosphorylated substrates for the corresponding kinase based on (red) a higher ratio than the positive control and (orange) a ratio value greater than a set threshold (see “Materials and methods”). c Kinase-target network connecting 65 positively phosphorylated substrates by a panel of eight kinases. d Venn graph of substrates targeted by CDK1/cycB1, CDK1/cycA2, and NEK2.
