Fig. 3: SUV39H1 overexpression reduced the frequency of leukemia stem cells in MLL-AF9-induced murine AML.

a, c Cell frequency analyses of c-Kit⁺ (a) and L-GMP (c) in bone marrow (BM) and spleen (SP) from moribund tertiary recipients using flow cytometry. n = 6, ***P < 0.001, Student’s t test. b, d Absolute cell number of c-Kit⁺ (b) and L-GMP (d) in BM and SP from moribund tertiary recipients. Leukemic cells were collected from femur, tibia and ilium (for BM) and whole SP (for SP), and dead cells were excluded using Trypan blue staining. n = 6, ***P < 0.001, Student’s t test. e, f Limiting dilution assay estimation of LSC frequency of P0 (e) and P1 (f) AML cells. Left panels: Logarithmic plot showing the percentage of non-responding recipients transplanted with different cell doses of eGFP⁺ P0 (e) and P1 (f) BM cells. Right panels: Table showing the number of recipients that developed leukemia and total number of recipients transplanted per cell dose. The chi-squared test was used. **P < 0.01, ***P < 0.001.