Fig. 4: The downregulation of LINC00261 in pancreatic cancer is caused by epigenetic modification. | Oncogene

Fig. 4: The downregulation of LINC00261 in pancreatic cancer is caused by epigenetic modification.

From: Epigenetic silencing of LncRNA LINC00261 promotes c-myc-mediated aerobic glycolysis by regulating miR-222-3p/HIPK2/ERK axis and sequestering IGF2BP1

Fig. 4

A A diagram illustrating primer design for BSP analysis of the promoter region of LINC00261. Methylation level in BSP region 1 using DNA samples from B a pancreatic cancer cell line and normal pancreatic ductal epithelial cells and from C primary pancreatic cancer tissue, adjacent normal tissue, and metastatic tissue. D MSP analysis using DNA samples from six pairs of primary pancreatic cancer tissue and adjacent normal tissue and four cases of metastasis. E LINC00261 expression in pancreatic cancer cell lines and normal pancreatic ductal epithelial cells by qRT-PCR and the corresponding methylation level by MSP. F The expression of LINC00261 in Panc1 and Mia-PaCa2 cells after treatment with 5-azacytidine (DNA methyltransferase inhibitor) for 24 and 48 h, respectively. The correlation analysis between EZH2 and LINC00261 using data from G TCGA database and H our center. I The expression of LINC00261 in Panc1 and Mia-PaCa2 cells after knockdown of EZH2. J Fold enrichment of EZH2 and H3K27me3 in the promoter region of LINC00261 detected by ChIP assay. K The enrichment fold of H3K27me3 in the promoter region of LINC00261 after knockdown of EZH2. The data are presented as the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01.

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