Fig. 7: LINC00261 decreases c-myc mRNA stability by sequestering IGF2BP1. | Oncogene

Fig. 7: LINC00261 decreases c-myc mRNA stability by sequestering IGF2BP1.

From: Epigenetic silencing of LncRNA LINC00261 promotes c-myc-mediated aerobic glycolysis by regulating miR-222-3p/HIPK2/ERK axis and sequestering IGF2BP1

Fig. 7

A Silver staining of LINC00261-associated proteins. One specific band (arrow) was excised and subjected to mass spectrometry. B Western blot of IGF2BP1 using protein samples enriched by biotinylated LINC00261, anti-LINC00261, and beads from Panc1 and Mia-PaCa2 cells. C Fold enrichment of LINC00261 in RNA samples precipitated by IGF2BP1 or IgG antibody in Panc1 and Mia-PaCa2 cells. The D mRNA and E protein levels of c-myc in Panc1 and Mia-PaCa2 cells with IGF2BP1 knockdown or overexpression. F The protein expression of c-myc in Panc1 and Mia-PaCa2 cells transfected with LINC00261 or cotransfected with LINC00261 and IGF2BP1. G Enrichment of IGF2BP1 measured using the biotinylated UTR region of c-myc in Panc1 and Mia-PaCa2 cells transfected with empty vector or vector with full-length LINC00261. The mRNA stability of c-myc in H Panc1 and I Mia-PaCa2 cells overexpressing LINC00261 or overexpressing LINC00261 and IGF2BP1 after treatment with actinomycin. The data are presented as the mean ± SD of three independent experiments. *P < 0.05. ns no significance.

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