Fig. 3: FOXO3a induces miR-29b-2 and miR-338 expression in breast cancer.
From: FOXO3a-driven miRNA signatures suppresses VEGF-A/NRP1 signaling and breast cancer metastasis
A Unsupervised clustering analysis showed differentially expressed miRNAs in MDA-MB-231/FOXO3a cells compared with control cells. B Venn diagram analysis examined overlapping miRNAs with differentially expressed miRNAs and predicted miRNAs targeting VEGF-A/NRP1 via TargetScan. C Expression levels of miR-29b-2, miR-388, and miR-211 in MDA-MB-231/FOXO3a and BT549/FOXO3a cells were quantified by qRT-PCR. **P < 0.01. D DNA sequence analysis revealed two FOXO3a-binding sites in the miR-29b-2 promoter region and a FOXO3a-binding site in the miR-338 promoter region. E A ChIP-qPCR assay was conducted to validate the binding of FOXO3a-binding sites to the promoter regions of miR-29b-2 and miR-388. *P < 0.05. F Luciferase reporter was performed to confirm the regulation of the promoter regions of miR-29b-2 and miR-338 by FOXO3a. **P < 0.01.
