Fig. 4: miR-29b-2 directly targets VEGF-A while miR-338 directly targets NRP1.
From: FOXO3a-driven miRNA signatures suppresses VEGF-A/NRP1 signaling and breast cancer metastasis
A Schematic diagram showing the predicted miR-29b-2 site in the human VEGF-A mRNA 3′UTR and the predicted miR-338 site in the human NRP1 mRNA 3′UTR. B Relative luciferase activity was analyzed in HEK293T cells cotransfected with miRNA mimics and luciferase reporter vectors (pLuc-3′UTR). C The expression of VEGF-A and NRP1 in MDA-MB-231 and BT549 cells transfected with miR-29b-2 or miR-338 was measured by Western blot. D The expression of VEGF-A and NRP1 in MDA-MB-231 and BT549 cells transfected with miR-29b-2 or miR-338 was measured by qRT-PCR. **P < 0.01.
