Fig. 5: miR-29b-2 and miR-338 synergistically inhibit breast cancer invasion and metastasis.
From: FOXO3a-driven miRNA signatures suppresses VEGF-A/NRP1 signaling and breast cancer metastasis
A The expression of miR-29b-2 and miR-338 in a data set of syngeneic cell lines with varying metastatic capabilities was measured by qRT-PCR. B The migratory capacity of MDA-MB-231 cells stably expressing miR-29b-2, miR-338, or miR-29b-2/miR-338 was determined by the wound-healing assay. C, D The invasion capacity of MDA-MB-231, BT549, and HCC1937 cells stably expressing miR-29b-2, miR-338, or miR-29b-2/miR-338 was determined by the transwell assay. *P < 0.05, **P < 0.01. E The expression of EMT markers in MDA-MB-231 and BT549 cells stably expressing miR-29b-2, miR-338, or miR-29b-2/miR-338 was measured by Western blot. F MDA-MB-231 cells stably expressing miR-29b-2, miR-338, or miR-29b-2/miR-338 were injected into the tail veins of nude mice and followed over 6 weeks. Representative H&E staining of lung sections is shown. G The numbers of lung micrometastases per section were counted and analyzed.
