Fig. 4: The UBE2T-RACK1 axis promotes the hyperactivation of the Wnt/β-catenin signaling pathway.
A, B The wild-type UBE2T plasmids or UBE2TC86A mutant plasmids were transfected into HEK-293T cells. Twenty-four hours after transfection, total cell lysates (B), cytoplasmic, or nuclear lysates (A) were analyzed by western blot with the indicated antibodies. C Correlational analysis between UBE2T and β-catenin immunohistochemistry (IHC) score in GC tissues. Spearman correlation coefficient = 0.7242; P < 0.0001. n = 155. D, E Mock or UBE2T plasmids were individually transfected into in wild-type or RACK1-deficient HEK-293T cells. Twenty-four hours after transfection, total cell lysates (E), cytoplasmic, or nuclear lysates (D) were analyzed by western blot with the indicated antibodies. F, G Wild-type and indicated lysine residual mutated RACK1 plasmids were individually transfected into HEK-293T cells with or without UBE2T. Twenty-four hours after transfection, total cell lysates (G), cytoplasmic or nuclear lysates (F) were analyzed by western blot with the indicated antibodies. H Schematic diagram of the mechanism that UBE2T drives gastric carcinogenesis.
