Fig. 2: ZNF263 acts as a transcriptional repressor of SIX3.
From: The EGFR-ZNF263 signaling axis silences SIX3 in glioblastoma epigenetically
a Putative ZNF263 binding motif in SIX3 promoter. b Experiments with TCGA and REMBRANDT showing the inverse correlation between the ZNF263 and SIX3 levels in glioma tissues. c Luciferase reporter assay showing that ZNF263 suppresses the transcriptional activity of SIX3 promoter. d ChIP-qPCR analysis showing that ZNF263 binds to SIX3 promoter. e EMSA showing that ZNF3 binds to SIX3 promoter in vitro. f Immunoblotting and (g) RT-qPCR analyses showing that ZNF263 suppresses SIX3 expression. *p < 0.05; **p < 0.01; ***p < 0.001.
