Fig. 4: MiR-590-5p functions as a tumor suppressor in GC by targeting YAP1.

a, b MiR-590-5p was successfully overexpressed or knocked down in GC cells. c MiR-590-5p overexpression suppressed GC cell proliferation. d MiR-590-5p knockdown promoted GC cell proliferation. e The potential target sites of miR-590-5p in YAP1. f Luciferase assay demonstrated miR-590-5p targeting both binding site 1 and binding site 2 of YAP1. g, h miR-590-5p inhibited YAP1 on both protein level and RNA level in GC cell. i Rescue experiments showed that the inhibition effect of miR-590-5p on GC cell proliferation can be rescued by YAP1 overexpression. j MiR-590-5p was downregulated in GC tissue in clinic samples. k YAP1 mRNA was upregulated in GC tissue in clinic samples. l YAP1 protein was upregulated in GC tissue in clinic samples. m MiR-590-5p expression showed negative correlation to YAP1 expression. *P < 0.05; **P < 0.01; ***P < 0.001 (paired t-test, two tailed).