Fig. 3: YY1-influenced DCBLD1 expression varied by rs17079281 genotype.

a Top panels: schematic of homozygous knockin of rs17079281 C allele. The sgRNA protospacers are underlined, the SNP position is indicated in red and the PAMs in green. Bottom panels: the knockin homozygous(C/C) genotype clones were confirmed by Sanger sequencing. b The relative expression level of DCBLD1 as determined by qRT-PCR in wild-type Beas2B cells (C/T) and the knockin clone (C/C). Results are shown as means ± SEM relative to β-actin levels and normalized to mean expression level in the knockin clone. The experiments are performed in three replicates. **P < 0.01 and P value is calculated with two-sided t tests. Immunoblot analysis of DCBLD1 protein in wild-type Beas2B cells(C/T) and the knockin clone (C/C). d CHIP assays are performed with control IgG or antibody to YY1 on the chromatin obtained from wild-type Beas2B cells (C/T) and the knockin clone (C/C), which are examined via qRT-PCR. Results are shown as means ± SEM and normalized to the positive control region from three independent experiments. **P < 0.01 and P value is calculated with two-sided t tests. e, f The expression level of DCBLD1 as determined by qRT-PCR (e) and western blotting (f) in wild-type Beas2B cells (C/T) and the knockin clone (C/C), which are transiently transfected with 1 μg or 2 μg of YY1 overexpression plasmid. Results are shown as means ± SEM relative to β-actin levels and normalized to control. The experiments are performed in three replicates. *P < 0.05 and P value is calculated with two-sided t tests.