Fig. 2: Microarray-based transcriptome analysis of the influence of chemokines on drug-promoted cellular dormancy entry and exit in LN229 GBM cells.

Cellular dormancy entry was promoted by stimulating with 500 µM TMZ or 0.5% (v/v) DMSO, respectively, with or without a chemokine mix containing 2 nM CXCL12, CXCL16, and CX3CL1 for ten days. The cells were incubated for a further 15 days without TMZ but with or without the chemokine mix (n = 3 biological replicates). Transcriptome analysis was performed using the ArrayXS® human 8 × 60 K microarray, and n-fold expression differences were displayed as log2-fold changes (log2FC) with a log2FC = 2 value indicating a 4-fold expression difference. All log2FC values between −1 and 1 were excluded, and only statistically significant values were enclosed. Venn diagrams for cellular dormancy entry (top) and exit (bottom) were prepared with each circle representing genes that had been identified as being significantly differentially expressed after TMZ-stimulation versus DMSO control, as well as in the TMZ versus DMSO + chemokines groups, respectively. Areas of overlap represent genes that are significantly differentially expressed in both groups. The numbers in the Venn diagrams indicate the number of genes in the particular Venn area. Complete gene lists including log2FC and p values of different Venn areas are given in Supplementary Tables 1 and 2.