Fig. 1: MMSET identified as a novel telomere-induced genomic instability regulator.
From: H3K36 dimethylation by MMSET promotes classical non-homologous end-joining at unprotected telomeres
a Experimental setup of the survival screen shown in (b). After infection with the retroviral shRNA-pools, cells were grown at the nonpermissive temperature (39 °C) to induce telomere uncapping for 12 days and returned to 32 °C for 14 days prior to staining with crystal violet. b Relative survival of TRF2ts MEFs infected with the indicated shRNA target gene pools (Unc = uncharacterized). After staining, crystal violet was extracted for quantification. Survival in control infected cells was used as reference (=1). c Survival assay in TRF2ts MEFs with deconvoluted shRNA-pool 7 used in (b). d Survival assay in TRF2ts MEFs infected with individual shRNAs targeting Mmset. e Growth curves of control or MMSET-depleted TRF2ts MEFs complemented with control or shRNA-resistant (RR) Flag-tagged MMSETRR and grown at 39 °C for the indicated days (data represent mean ± s.d. of a technical triplicate).
