Fig. 7: Replication fork stalling and DNA fragmentation upon combination treatment. | Oncogene

Fig. 7: Replication fork stalling and DNA fragmentation upon combination treatment.

From: Resistance to the CHK1 inhibitor prexasertib involves functionally distinct CHK1 activities in BRCA wild-type ovarian cancer

Fig. 7

a DNA fiber assay to measure replication fork stalling was performed on cells treated with CldU (20 min), IdU (10 min) followed by the addition of Gem (50 nM) or Prex (50 nM) or both for a further 2 h in the presence of IdU. The ratio of nascent IdU labeled strands to CldU labeled strands are plotted as a measure of stalled replication forks. b, c Alkaline comet assay to quantify DNA fragmentations in cells treated with Prex or Gem or in combination. At least 100 cells events were quantified using CometScore© software. Percentage of tail DNA is plotted. Each experiment was repeated at least twice. *P < 0.05; **P < 0.01; ***P < 0.001; NS not significant.

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