Fig. 2: LNM-GC-derived exosomes exhibit an enhanced ability to educate BM-MSCs relative to the ability of primary GC-exosomes. | Oncogene

Fig. 2: LNM-GC-derived exosomes exhibit an enhanced ability to educate BM-MSCs relative to the ability of primary GC-exosomes.

From: Lymph node metastasis-derived gastric cancer cells educate bone marrow-derived mesenchymal stem cells via YAP signaling activation by exosomal Wnt5a

Fig. 2

a–d Characterization of AGS- and SGC-7901-exosomes. a Representative transmission electron microscopy (TEM) images (magnification, ×60,000; scale bars, 100 μm). b Size distribution shown by nanoparticle tracking analysis (NTA). c Flow cytometry analysis of CD81 expression. d Western blotting analysis of CD63 and CD9 expression. e–i The ability of AGS- and SGC-7901-exosomes to educate BM-MSCs was evaluated. e α-SMA expression in BM-MSCs (magnification, ×200; scale bars, 50 μm). f Quantification of migration, invasion, and tubule formation. g–i In vivo analysis of tumor-promoting properties of BM-MSCs. g The draining popliteal lymph nodes (LNs) from each group were harvested and imaged. h Weight of popliteal LNs. i Immunohistochemistry analysis of pan-cytokeratin (AE1/AE3) expression in popliteal LNs. Representative images from each group are shown (magnification, ×100, scale bars, 100 μm; magnification, ×400, scale bars, 20 μm). Data are expressed as the mean ± SD of three independent experiments. Statistical significance was calculated using one-way ANOVA followed by Tukey’s test. ***P < 0.0001; **P < 0.01; ns, non significant.

Back to article page