Fig. 5: Serum exosomes, isolated from LNM-GC patients educate BM-MSCs, and circulating exosomal Wnt5a content is associated with LNM. | Oncogene

Fig. 5: Serum exosomes, isolated from LNM-GC patients educate BM-MSCs, and circulating exosomal Wnt5a content is associated with LNM.

From: Lymph node metastasis-derived gastric cancer cells educate bone marrow-derived mesenchymal stem cells via YAP signaling activation by exosomal Wnt5a

Fig. 5

a–d Characterization of serum exosomes isolated from healthy participants (H), GC patients without LNM (NM), and GC patients with LNM (M). a Representative TEM images of exosomes (magnification, ×60,000; scale bars, 100 μm). b Size distribution of exosomes analyzed using NTA; c Detection of CD81 expression by flow cytometry. d Western blotting analysis of CD63 and CD9 expression. e, f Comparison of BM-MSC-educating capacity among exosomes from the three groups described above. e Detection α-SMA expression in BM-MSCs (magnification, ×200; scale bars, 50 μm). f Analysis of tumor-promoting properties of BM-MSCs. g, h Wnt5a expression in exosomes isolated from the pooled sera was detected by western blotting. g Representative image. h Fold changes in relative gray values of Wnt5a; i, j Serum concentration of exosomal Wnt5a was detected using ELISA. i Serum concentration of exosomal Wnt5a in clinical serum samples (20 individuals per group). j Wnt5a concentration in serum samples from the LNM model described in Figs. 2, 4. Data are presented using means ± SD of three independent experiments. Statistical significance was determined using one-way ANOVA followed by Tukey’s test. ***P < 0.0001; **P < 0.01; *P < 0.05; ns, non significant.

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