Fig. 4: The phosphorylation of ANXA2 at Tyr24 by YES1 increases ANXA2 nuclear distribution.
From: EphA2–YES1–ANXA2 pathway promotes gastric cancer progression and metastasis
A YES1 co-immunoprecipitation with ANXA2 or ANXA2 co-immunoprecipitation with YES1 in indicated GC cells. B Western blot for the expression of indicated proteins in control and YES1-OE MGC-803 cells, or control and YES1-KD AGS cells. C Recombinant human His-ANXA2 was incubated with the absence or presence of recombinant human active GST-YES1 at 30 °C for 30 min. The reaction mixtures were analyzed by immunoblotting with anti-p-ANXA2 (Tyr24), ANXA2, and YES1 antibodies. D Western blot for the expression of indicated proteins in control, YES1-OE and YES1-KD cells transiently transfected with control or EphA2 overexpression plasmid for 48 h. E Western blot for the expression of indicated proteins in MGC-803 cells transiently transfected with control or WT-YES1, YES1-Y426F, and YES1-Y537F expression plasmid for 48 h. F Western blot for the expression of indicated proteins in cytoplasmic and nuclear extracts of control and YES1-OE MGC-803 cells or control and YES2-KD AGS cells. G Immunofluorescent staining of indicated proteins in control and YES1-OE MGC-803 cells or control and YES1-KD AGS cells. H Western blot for the expression of indicated proteins in cytoplasmic and nuclear extracts of ANXA2-KD cells transiently transinfected with control, ANXA2-WT or MT-ANXA2-Y24F expression plasmid for 48 h.
