Fig. 1: CIN of the cervical squamous epithelium in OVX-treated WAPL Tg mice injected with E2.
From: WAPL induces cervical intraepithelial neoplasia modulated with estrogen signaling without HPV E6/E7
A HE stain and IHC analysis for Keratin 14 (KRT14) in cervical squamous epithelium of Wt, HPV E6/E7 KI and WAPL Tg mice at 3 and 6 months after OVX. The mice were divided into two groups, the E2-injected group and non-E2-injected group. The scale bars represent 50 μm. B Expression of Ki-67 and P16 in cervical squamous epithelium of E2-injected Wt, HPV E6/E7 KI and WAPL Tg mice at 6 months after OVX. Left panels show IHC analysis for Ki-67 and P16. Insets show higher magnification images. The scale bars represent 50 μm. Right panels show the graphs representing the rates of Ki-67 and P16 positive cells in the cervical squamous epithelium. C Comparison of WAPL protein expression in the uterine cervix between E2-injected HPV E6/E7 KI and WAPL Tg mice at 6 months after OVX by western blot analysis. The data were obtained from 6 mice for each group, and the representative images are shown. Error bars on the graphs in B represent SD of the mean. Statistical significance was calculated by Student t test. *p < 0.05. n.s. no significant difference.
