Fig. 2: Immunohistochemical and ultrastructural localization of FMRP at the leading edge in iCCA cells. | Oncogene

Fig. 2: Immunohistochemical and ultrastructural localization of FMRP at the leading edge in iCCA cells.

From: Fragile X mental retardation protein in intrahepatic cholangiocarcinoma: regulating the cancer cell behavior plasticity at the leading edge

Fig. 2: Immunohistochemical and ultrastructural localization of FMRP at the leading edge in iCCA cells.

a Representative images of a IHC for FMRP in an iCCA sample, showing FMRP staining mainly localized beneath plasma membrane of iCCA cells, with a strong enhancement of signal towards the basal lamina (upper panels, black arrowhead). Cortactin staining exhibits a similar intracellular pattern (lower panels, white arrowhead). Calibration bars 20 µm. Original magnification 20× (left panels), high power fields 100× (right panels). b Ultrathin sections of HuCCT cellular pellets were subjected to immunogold electron microscopy with specific FMRP and Cortactin antibodies. The box in left panel indicates the high-power field in right panel. 25 nm gold particles (FMRP, white arrowheads) and 15 nm gold particles (Cortactin, black arrows) colocalized at the base of membrane protrusions of HuCCT cells. The white arrows indicate ribosomes densities. Nu Nucleus, Cyt Cytoplasm. Left panel: original magnification ×18,500. Scale bar: 1 μm. Right panel: original magnification ×37,000. Scale bar: 500 nm.

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