Fig. 4: FOXM1 is required for DKK1 expression.
A Lysates of S2-CP8 cells, HPAF-II cells, TE-1 cells, TE-5 cells, and TE-8 cells stably expressing control shRNA or FOXM1 shRNAs were probed with the indicated antibodies. Clathrin was used as a loading control. B The mRNA levels of DKK1 in various cells used in Fig. 4A were measured by quantitative RT-PCR and normalized to GAPDH. The results are shown as fold-changes compared to control shRNA-expressing cells and are expressed as meansโยฑโSD from three independent experiments. C Lysates of S2-CP8 and TE-5 cells stably expressing control shRNA, or cells stably expressing FOXM1 shRNA with control vector (โ), FLAG-FOXM1c, or FLAG-FOXM1cฮNLS were probed with the indicated antibodies. Clathrin was used as a loading control. D The mRNA levels of DKK1 in the S2-CP8 cells and TE-5 cells used in Fig. 4C were measured by quantitative RT-PCR and normalized to GAPDH. The results are shown as fold-changes compared to control shRNA expressing cells and are expressed as meansโยฑโSD from three independent experiments. E and G Lysates of S2-CP8 cells stably expressing control shRNA, FOXM1 shRNA, or FOXM1 shRNA and T7-FOXM1b (E) and Capan-1 cells stably expressing control vector, FLAG-FOXM1c, or FLAG-FOXM1cฮNLS (G) were probed with the indicated antibodies. Clathrin was used as a loading control. F and H The mRNA levels of DKK1 in the S2-CP8 cells used in Fig. 4E (F) and Capan-1 cells used in Fig. 4G (H) were measured by quantitative RT-PCR and normalized to GAPDH. The results are shown as fold-changes compared to cells expressing control shRNA or control vector. The results are expressed as meansโยฑโSD from three independent experiments. *Pโ<โ0.05; **Pโ<โ0.01 (Studentโs t test).
