Fig. 5: RNF141 involves in tumorigenesis in vivo.
From: RNF141 interacts with KRAS to promote colorectal cancer progression
A–E Knockdown of RNF141 suppressed tumor growth in vivo. Images of LV-sh-NC or LV-sh-RNF141-transfected cell xenograft tumor models (A); Tumor growth curve (B) and average weight (C) were recorded and counted. The RNF141 protein level in xenograft tumors from LV-sh-NC or LV-sh-RNF141 group was confirmed by Western blot (D). The IHC staining (E) was applied to determine the expression of RNF141 and PCNA in LV-sh-NC or LV-sh-RNF141-transfected cell xenograft tumor. Scale bar, 50 μm. F–J RNF141 overexpression facilitated tumor growth in vivo. Xenograft tumor models of HCT116 cells transfected with LV-NC or LV-RNF141 were displayed (F), Tumor growth curve (G) and average weight (H) were recorded and counted. Western blot was performed to confirm RNF141 protein level in xenograft tumors from LV-NC or LV-RNF141 group (I). The IHC staining (J) was applied to determine the expression of RNF141 and PCNA in LV-NC or LV-RNF141-transfected cell xenograft tumor. Scale bar: 50 μm. Data represented mean ± SD. n = 6 each group. **P < 0.01, ***P < 0.001.
