Fig. 7: ZDHHC1-mediated p53 palmitoylation is required for suppressing tumor cell growth.
From: Cancer cells escape p53’s tumor suppression through ablation of ZDHHC1-mediated p53 palmitoylation
A CCK8 proliferation assay of MCF7 and HONE1 cells. B Cell cycle analysis of MCF7 and HONE1 cells. See also Fig. S5A. C Flow cytometric analysis of apoptotic MCF7 and HONE1 cells expressing ZDHHC1 or ZDHHC1C164A. See also Fig. S5B. D CCK8 proliferation assay of MCF7 cells treated with 2Brp. E CCK8 proliferation assay of HONE1 cells treated with 2Brp. F CCK8 proliferation assay of H1299 cells ectopically expressing different variants of p53. G Flow cytometric analysis of apoptotic H1299 cells ectopically expressing different variants of p53. See also Fig. S6A–C. H mRNA expression of P21 in H1299 cells by qRT-PCR. I mRNA expression of BAX in H1299 cells by qRT-PCR. Values represent mean ± SEM. **p < 0.01, ***p < 0.001.
