Fig. 5: DEGS2 promotes CRC proliferation and migration. | Oncogene

Fig. 5: DEGS2 promotes CRC proliferation and migration.

From: M6A methylation of DEGS2, a key ceramide-synthesizing enzyme, is involved in colorectal cancer progression through ceramide synthesis

Fig. 5: DEGS2 promotes CRC proliferation and migration.The alternative text for this image may have been generated using AI.

a DEGS2 mRNA levels in different CRC cell lines were verified by RT-qPCR. CCD-841 was selected as the control. b The DEGS2 overexpression and knockdown efficiency was verified at the mRNA (left panel) and protein levels (right panel) in HCT116 cells by qRT-PCR and WB assays, respectively. c CCK-8 assay of DEGS2-overexpressing and DEGS2 knockdown HCT116 cells. d Knockdown of DEGS2 impaired the colony formation ability, and overexpression of DEGS2 enhanced colony formation in HCT116 cells (left panel); quantification of the colony formation assay results (right panel). e The wound healing rate of cells transfected with wild-type (control) or DEGS2-overexpressing lentivirus cells, or DEGS2 knockdown shRNA was recorded (left) and quantitatively analyzed (right). f Representative images (left panel) and quantification (right panel) of the cell migration and invasion assay results for DEGS2-overexpressing and DEGS2-knockdown HCT116 cells transfected with DEGS2 shRNAs, lentivirus or their corresponding controls. Cells were allowed to invade for 24 h. g The colony formation, wound healing, and cell migration and invasion abilities of DEGS2-WT and DEGS2-mutant (GACT to GGCT in CDS motif) HCT116 were recorded (left) and quantitatively analyzed (right).

Back to article page