Fig. 1: Mammary-specific ablation of β1 integrin inhibits tumour initiation, tumour progression to invasive carcinoma and lung metastasis. | Oncogene

Fig. 1: Mammary-specific ablation of β1 integrin inhibits tumour initiation, tumour progression to invasive carcinoma and lung metastasis.

From: Emergence of β1 integrin-deficient breast tumours from dormancy involves both inactivation of p53 and generation of a permissive tumour microenvironment

Fig. 1

a Epithelial transformation (tumour initiation) after 2 week-post Dox induction in MIC mice carrying either wild type (MIC WT) or floxed β1 integrin (MIC β1KO) alleles. Upper panel shows whole mount analysis of mammary gland number 4. Scale bar is 5 mm. 28% of MIC β1KO mice did not exhibit epithelial transformation (similar to MTB control glands). Lower panel shows H&E images of hyperplastic lesions that are not yet invasive (MIN). Scale bars is 3 mm. MTB control mice represents non-transformed glands. b Quantification of epithelial area normalised to total gland area using H&E images in a. Data: mean ± SEM, two-tailed Student’s t-test. c Percent of tumour-free survival. P value was calculated between MIC WT and MIC β1KO cohort using Lox-rank Mantel Cox test. d Representative H&E images of lungs collected at end-burden tumour mass. Green outline indicates visible metastases. Scale bars are 1 cm (upper panel) and 100 μm (lower panel). e Quantification of lung metastatic burden including percentage of mice with metastasis (MIC WT n = 19, MIC β1KO n = 14), number and total area of lung metastases per animal. Data: mean ± SEM, two-tailed Student’s t test.

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