Fig. 2: Silence of CD147 potentiates paclitaxel-induced cytotoxicity.
From: CD147 supports paclitaxel resistance via interacting with RanBP1
A Western blot analysis of CD147 expression in SK-OV-3 cells. B Apoptosis analysis of SK-OV-3 cells by flow cytometry. C Representative images of TUNEL staining in SK-OV-3 cells. Scale bar = 20 μm. The graph shows quantification of the percentage of TUNEL positive cells. D Western blot analysis of the indicated proteins in SK-OV-3 cells. The graphs show semi-quantitative analysis of relative cleaved PARP and cleaved caspase-3 expression. The p values in (C and D) were determined by using two-tailed Student’s t test. E Cell cycle distribution of SK-OV-3 cells. F Schematic representation of subcutaneous cancer xenografts, as well as the schedule of paclitaxel administration in the nude mouse model. n = 6 for each group. G Nude mice with subcutaneous SK-OV-3 xenografts were imaged in a Xenogen IVIS 200 system. H Representative images of immunochemistry staining of CD147 and Ki67 on subcutaneous SK-OV-3 xenografts. Scale bar = 200 μm. The graph summarizes the IHC score of Ki67 and the p value was determined by using Mann–Whitney U test.
