Fig. 5: The intracellular domain of CD147 is indispensable for CD147-regulated paclitaxel resistance.

A Schematic representation of the CD147 constructs. B Analysis of apoptosis by flow cytometry. CD147 knockdown SK-OV-3 cells were transfected with CD147^WT or CD147^ΔICD. Cells were treated with PBS or 0.1 μM paclitaxel. C The graph shows quantification of the percentage of TUNEL positive cells. D Western blot analysis of the indicated proteins in CD147 knockdown SK-OV-3 cells. Cells were transfected with CD147^WT or CD147^ΔICD and treated with or without 0.1 μM paclitaxel. The graphs show semi-quantitative analysis of protein expression. E Schematic representation of the CD147 constructs. F Analysis of apoptosis by flow cytometry. CD147 knockdown SK-OV-3 cells were transfected with CD147^WT, CD147^ΔECD or CD147^ICD. Cells were treated with PBS or 0.1 μM paclitaxel. G The graph shows quantification of the percentage of TUNEL positive cells. H Western blot analysis of the indicated proteins in CD147 knockdown SK-OV-3 cells. Cells were transfected with CD147^WT, CD147^ΔECD or CD147^ICD and treated with or without 0.1 μM paclitaxel. The graphs show semi-quantitative analysis of protein expression. The p values in (C–D), (G–H) were determined by two-tailed Student’s t test.