Fig. 6: CD147ICD interacts with RanBP1.
From: CD147 supports paclitaxel resistance via interacting with RanBP1
A MS/MS confirmation of a RanBP1 peptide. B Western blot analyses of endogenous CD147 co-immunoprecipitated with endogenous RanBP1 in SK-VO-3 cells. IgG was used as a control antibody for immunoprecipitation. C Representative images of immunofluorescent staining of endogenous CD147 and RanBP1 in A549 cells. Scale bar = 10 μm. D SPR diagram of RanBP1 protein bound to CD147ICD. The KD values were calculated by the the ProteOn Manager Software. E–F The interaction between the indicated constructs was analyzed with FRET. The color bar represents the FRET ratio. Scale bar, 10 μm. The graphs show FRET efficiencies. The p values were determined by two-tailed Student’s t test.
