Fig. 4: ADRB blockade by propranolol reduces intratumoral MDSCs and upregulates PDL1 expression on tumor associated macrophages (TAMs). | Oncogene

Fig. 4: ADRB blockade by propranolol reduces intratumoral MDSCs and upregulates PDL1 expression on tumor associated macrophages (TAMs).

From: Blockade of beta-adrenergic receptors reduces cancer growth and enhances the response to anti-CTLA4 therapy by modulating the tumor microenvironment

Fig. 4

Single cell suspensions were made from excised MCA205 tumors in immune competent mice and T cell deficient nude mice at the experimental endpoint and analyzed by flow cytometry (n = 3–5). A, B Representative flow cytometry dot plot (A) and quantification of MDSCs (CD11b + F4/80- GR1 + ) (B) in the TME of tumors from control and propranolol (PRO) groups in immune competent mice. C Quantification of TAMs (CD11b + F4/80+) in samples from control and propranolol treated immune competent mice. D, E, Representative histograms and quantifications of mean fluorescence intensity (MFI) of PDL1 on TAMs from immune competent mice (D), and T cell deficient nude mice (E). MFI quantification of MHC II (F) and CD206 (G) expression on TAMs in immune competent mice. Multiple t tests with Bonferroni correction for multiple comparison were used for statistical testing. *p < 0.05, **p < 0.01. Mean ± SEM are depicted.

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