Fig. 7: In vitro and in vivo effects of GKN1 protein. | Oncogene

Fig. 7: In vitro and in vivo effects of GKN1 protein.

From: De novo expression of gastrokines in pancreatic precursor lesions impede the development of pancreatic cancer

Fig. 7

A Viability of Panc-02 cells was assessed by MTT colorimetric assay after 24 h of incubation with conditioned media containing GKN1 protein. B β-Galactosidase staining on Panc-02 cells after incubation with GKN1 or control conditioned medium. Representative pictures of compared groups are shown for the 72 h time point. Expression profile of senescence genes such as p16 and p21 was performed via qPCR analysis on Panc-02 cells treated with conditioned media with or without GKN1. Gene expression was analyzed over 24, 48, and 72 h. C Migration assay of Panc-02 cells after 24 h of incubation with GKN1 or control conditioned medium. Representative pictures at 5x magnification show migration over 1, 10, and 24 h time period (scale bars 200 μm). D In vitro transdifferentiation of isolated acinar cells from KC mice, treated with conditioned media containing GKN1. E Subcutaneous tumor development over 19 days after injection of either Panc-02-empty vector or Gastrokine1+ Panc-02 cells.

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