Fig. 1: MTX resistance is associated with large scale proteomics changes in choriocarcinoma cells.
From: ATR and CDK4/6 inhibition target the growth of methotrexate-resistant choriocarcinoma
A JEG3 and JEG3R cells were treated with increasing concentrations of MTX and cell survival determined by Crystal Violet staining. B DHFR was detected from lysates from exponentially growing JEG3 and JEG3R cells using Western blotting. Detection of β-Tubulin was used as a loading control. Representative blots of three biological replicates. C JEG3R cells silenced or not for DHFR expression were treated with increasing concentrations of MTX and cell survival determined by Crystal Violet staining. Insert: qPCR for DHFR demonstrates efficient target down regulation. D, E Comparative network generated by Reactome FI under Cytoscape from the SILAC-based total proteomics analysis of JEG3 and JEG3R cells. The network was further fragmented into 13 modules based on GO biological processes annotation (D). E GO biological processes associated with the corresponding network modules. The number of nodes and linkers for each GO processes category is shown. Nodes; proteins detected by MS/MS. Linkers; additional nodes introduced by Reactome FI to maximise network connectivity. A–C Data are normalised mean ± SEM.
