Fig. 1: Expression of IL-36 family members is increased in colon cancer. | Oncogene

Fig. 1: Expression of IL-36 family members is increased in colon cancer.

From: IL-36 signalling enhances a pro-tumorigenic phenotype in colon cancer cells with cancer cell growth restricted by administration of the IL-36R antagonist

Fig. 1

A RNA was extracted from human colon cancer tissue and adjacent non-tumour tissue (n = 24), and gene expression of IL-36α, IL-36β, IL-36γ, IL-36RN and IL-36R were determined by qRT-PCR. B Paraffin-embedded human colon cancer tissue and adjacent non-tumour tissue (n = 66) were immunostained using anti-IL-36α, anti-IL-36β, anti-IL-36γ or anti-IL36R. Representative images are shown (mag = 40X; Scale 100 µm). C Affymetrix microarray data from the Gene Expression Omnibus (GSE68468) was analysed for IL-36R expression in normal colon mucosa, polyp tissue and colon cancer tissue. D RNA was extracted from colon cancer cell lines and murine bone marrow (BM)-derived macrophages and dendritic cells (DC), normalised, and expression of IL-36R analysed by RT-PCR. Statistical analysis was performed by Student T Test (A) and One-way ANOVA (C). p < 0.05, **p < 0.01, ***p < 0.001.

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