Fig. 6: CSDE1 interacts with RNAPII and Rac1 mRNA. | Oncogene

Fig. 6: CSDE1 interacts with RNAPII and Rac1 mRNA.

From: Reduced miR-371b-5p expression drives tumor progression via CSDE1/RAC1 regulation in triple-negative breast cancer

Fig. 6

A Relative mRNA expression of Rac1 in CSDE1 siRNA-treated MDA-MB-231 and Hs578T cells. Data are presented as mean ± SD with 3 biological replicates. B, C Interaction of CSDE1 Flag with RNAPII p-CTD (ser2, ser5) in HEK293T cells transfected with control or CSDE1-Flag-tagged vector. D Interaction of endogenous CSDE1 with RNAPII p-CTD (ser2, ser5) in MDA-MB-231 cells. E The enrichment of Rac1 mRNA was measured via qPCR after RNA pull-down by IgG, Flag, RNAPII p-CTD (ser2), and RNAPII p-CTD (ser5) antibodies in HEK293T cells transfected with control or CSDE1-Flag-tagged vector. A 10% input was used for measurement in E. Data are presented as mean ± SD with 3 technical replicates. *p < 0.05, ***p < 0.005.

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